Fig. 4: Analysis of a frequent inversion on chromosome 2H.

a, A PCA showing the localization of inversion carriers in the diversity space of global domesticated barley. The correspondence of PCA coordinates to correlates of population structure is shown in Extended Data Fig. 1. Red dots denote carriers of the inverted haplotype (n = 87) in a panel of 200 domesticated varieties of barley. b, PCA for a diversity panel comprising 200 domesticated (red and green dots) and 100 wild varieties of barley (blue dots). SNP markers detected in whole-genome shotgun data and located in the inverted regions were used. c, Schematic of the inverted region. The HvCEN gene is closest to the breakpoint that is distal in Morex (distance of 449 kb) and proximal in Barke (distance of 433 kb) assemblies. A total of 46 and 44 high-confidence (HC) genes were annotated in the Morex and Barke assemblies, respectively. The yellow arrows (not drawn to scale) mark the positions of PCR primers to probe for the presence of the inversion (Supplementary Fig. 2c).