Fig. 5: Investigation of lipid-metabolism genes in breast cancer brain metastasis.
a, A schematic of an in vivo CRISPR screen investigating relative gene fitness in brain metastasis outgrowth. b, Volcano plot showing the result of a mini-pool in vivo CRISPR screen targeting 29 lipid-metabolism-related genes. Thirteen genes scored at FDR < 0.05, with selective hits highlighted. c, Individual gene validation of six hits by intracranial injection of JIMT1 edited cells. Cell outgrowth in brain metastasis was monitored by real-time BLI. Two independent guides per gene were tested, with one guide per-mouse. d, BLI and quantification of relative fold change in metastasis load in the organs of mice receiving intracardiac injection of wild-type (WT) or SREBF1-knockout (KO) JIMT1 cells. Data are mean ± s.e.m. Each group contains five mice. e, BLI and quantification of relative fold change in brain metastasis load in mice receiving intracarotid injection of wild-type or SREBF1-KO JIMT1 cells. Data are mean ± s.e.m. n = 7 (wild-type) and n = 8 (knockout) mice.
