Extended Data Fig. 2: Using PRISM cell line pools for metastatic potential profiling.
a, Optimizing the workflow of metastatic potential mapping using PRISM. A PRISM pool of 25 cell lines was used for testing the need of GFP labelling and cancer cell purification. The barcode abundance altered compared to the unlabelled population after GFP labelling as shown by the pie chart. b, A detailed line-by-line view of barcode abundance before and after GFP labelling. The unlabelled cell pool had more even distribution. Post labelling, several lines showed noticeable dropout, but all lines were detectable. c, Scatter plot comparing barcode enrichment after normalizing to the pre-injected input from the two experiments. Pearson’s correlation coefficient and its test P value are presented. Strong positive correlation is observed, with the exception of one cell line U2OS. d, Quality control of MetMap500 and MetMap125 datasets showing initial barcode abundance in the pre-injected populations. MetMap500, 1 large pool containing 498 cell lines was profiled, with 10 cell lines showing low initial abundance. These 10 cell lines were not detected in any in vivo sample, and were excluded from subsequent analysis. MetMap125, 5 pools of 25 cell lines were profiled separately and data were combined for analysis. e, Quality control of MetMap500 and MetMap125 datasets showing scatter plots of raw barcode abundance from in vivo organs versus the data normalized to the pre-injected input (in d). A strong linear relationship was observed.
