Extended Data Fig. 2: Anatomical characterization of vpoDNs.
From: Neural circuit mechanisms of sexual receptivity in Drosophila females
a, Confocal images of brains and ventral nerve cords from female and male flies carrying vpoDN-SS1, vpoDN-SS2, or vpoDN-SS3, and UAS-myrFLAG, stained with anti-FLAG to reveal membranes of targeted neurons (green) and mAb nc82 to reveal all synapses (magenta). One pair of vpoDNs are labelled in females but not in males. Scale bar: 100 μm. b, Confocal images of female brains showing the co-labelling of vpoDNs with dsx-LexA but not fru-LexA. Scale bar: 20 μm. c, Confocal images of female brains showing the expression of ChAT, GAD1, and vGluT in vpoDN (labelled by Halo tag, arrows), as revealed by FISH. Scale bar: 10 μm. Representative images are shown from at least 5 independent samples examined in each case.
