Extended Data Fig. 6: Flexibility of SmM tail ribbon.
a, Representative class averages showing flexibility of the tail of myosin molecules (Supplementary Video 7), after alignment of the heads. Numbers at lower left indicate the chord angle of the tail at a position 29.7 nm along the tail from its emergence point from the head (taken to be the fulcrum). The angle is defined in relation to the path of the tail between bend 2 and the fulcrum, see the cartoon at the right. 4895 face view particles were classified into 50 classes of which 5 are shown to represent the range of motion of the tail. b, Cumulative frequency plot of tail angles for myosin with fitted Gaussian curve, yielding a variance value of 34°. Scale bar in a: 20 nm.
