Extended Data Fig. 2: Confirmation of the purity and identity of recombinant MmfR and in vivo assay for induction of methylenomycin production in S. coelicolor.
From: Molecular basis for control of antibiotic production by a bacterial hormone
a, Left, analysis of purified recombinant His6-MmfR by SDS–PAGE. MWM, molecular weight marker. Right, mass spectrometry analysis: measured (top) and deconvoluted (bottom) mass spectra of His6-MmfR. Calculated mass, 27,835.5 Da. b, In vivo assay for induction of methylenomycin production upon addition of increasing amounts of MMF1 to growing mycelia of the MMF non-producing strain S. coelicolor W81. Methylenomycin production was detected by growth inhibition of the methylenomycin-sensitive strain S. coelicolor M145.
