Fig. 1: The epigenomic landscape of cortical projection neurons.
From: Epigenomic diversity of cortical projection neurons in the mouse brain
a, Schematics of the epi-retro-seq workflow. SC, superior colliculus; MY, medulla; STR, striatum; TH, thalamus. All brain atlas images were created based on Wang et al.25 and ©2017 Allen Institute for Brain Science. Allen Brain Reference Atlas. Available from: http://atlas.brain-map.org. b–d, Two-dimensional t-SNE of 11,827 cortical neuron nuclei on the basis of mCH levels in 100-kb genomic bins, coloured by subclass (b), the source of neurons (c), or their projection target (d). Inh, inhibitory; NP, near-projecting; CLA, claustrum. e, Neighbour enrichment scores of cells categorized by subclass (n = 11,827), source (n = 11,827), target (n = 10,396) and replicate (n = 11,638). f, The distribution across cell subclasses of neurons that projected to each IT (left) or ET (right) target. g, AUROC of distinguishing between source pairs or target pairs computed for IT and ET neurons on the basis of gene body mCH (n = 73, 88, 32 and 41; from left to right). For all box plots, centre line denotes the median; box limits denote first and third quartiles; and whiskers denote 1.5 × the interquartile range.
