Addendum to: Nature https://doi.org/10.1038/s41586-018-0671-4Published online 31 October 2018

In our original Article, we developed a phototriggerable derivative of the actin disruptor cytochalasin D, denoted photo cytoD or Nvoc-CytoD, to show that a local perturbation of the actin cortex increases cell area (Extended Data Fig. 8 of the original Article). Through direct visualization, we showed that the F-actin cytoskeleton of cells that were incubated with Nvoc-CytoD (50 μM) was rapidly disrupted upon light exposure (Extended Data Fig. 8c). Cells incubated with Nvoc-CytoD without light exposure, and cells exposed to light without Nvoc-CytoD, did not show changes in their cytoskeleton or in cell area (Extended Data Fig. 8a–d). A detailed characterization of the synthesis and chemical validation of Nvoc-CytoD was not included in our original Article, and we would like to provide it here.

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