Extended Data Fig. 4: Map3k2 in intestinal stromal cells protects mice from DSS-induced colitis in vivo.

a, Representative flow cytometry plots showing the percentages of CD326⁺ intestinal epithelial cells (IECs), CD45⁺ leukocytes, GP38⁺CD90⁻ intestinal mesenchymal stromal cells, and GP38⁺CD90⁺ intestinal mesenchymal stromal cells in the colon of wild-type mice (n = 4 mice). b, c, qRT–PCR of Rspo1 (b) and Map3k2 expression (c) in the indicated cell types described in a. d, Representative flow cytometry plots and histograms showing percentages of tdTomato⁺ cells in IECs, CD45⁺ leukocytes, lymphatic endothelial cells (LECs), blood endothelial cells (BECs), GP38⁺ cells, and GP38⁻ cells from the colonic lamina propria of Col1a2-cre^ERT2Rosa26-loxP-stop-loxP-tdTomato mice as indicated. e, qRT–PCR of Map3k2 expression in CD45⁺ leukocytes, IECs, CD31⁺ endothelial cells, and GP38⁺ intestinal mesenchymal stromal cells from the colons of tamoxifen-treated Map3k2^fl/fl and Col1a2-cre^ERT2Map3k2^fl/fl mice (n = 3 mice per group). f, g, Average stool scores (f) and colon lengths (g) of DSS-treated Map3k2^fl/fl and Col1a2-cre^ERT2Map3k2^fl/fl mice (n = 3 mice per group) that had been previously induced with tamoxifen. h, Left, H&E-stained colons from mice in f. Right, quantification of the corresponding histology scores. Scale bars, 100 μm. i, qRT–PCR of Il6 expression in the colons (n = 3) of mice in f. Data presented as mean ± s.e.m. Numbers in plots are P values calculated by two-tailed unpaired Student’s t-test.

Source data