Extended Data Fig. 5: CD90 is highly expressed in LECs and moderately expressed in stromal cells.
From: MAP3K2-regulated intestinal stromal cells define a distinct stem cell niche
a, Lgr5-EGFP-creERT2 colon immunostained for CD90 (cyan), GP38 (red) and DAPI (blue). Box in left image (sub-crypt) area is expanded in the second image, and shown on cyan and red single channels, respectively, in the third and fourth images. Results are representative of three independent experiments. Scale bars, 30 μm. b, Volumetric reconstruction of whole-mount imaging of wild-type mouse colon stained with anti-CD90 (cyan) and anti-CD326 (red) antibodies. Results are representative of two independent experiments. c, Wild-type mouse colon immunostained for CD90 (green) and LYVE1 (red). Left, merged image. Results are representative of three independent experiments. Scale bars, 30 μm. d, Representative flow cytometry plots of IECs, CD45+ leukocytes, LECs, BECs, GP38+ stromal cells, and GP38− cells from wild-type colonic lamina propria. The numbers show the percentages of the indicated cell populations from the respectively gated areas. e, Histograms of CD90 expression in the indicated cell types described in d. Results are representative of three independent experiments.
