Extended Data Fig. 4: Phylogenetic affiliation of ‘Ca. A. ciliaticola’ within the Gammaproteobacteria.
From: Anaerobic endosymbiont generates energy for ciliate host by denitrification
16S rRNA gene sequence-based maximum-likelihood phylogenetic tree of ‘Ca. A. ciliaticola’ and other members of the Gammaproteobacteria. The circular metagenome-assembled genome-derived 16S rRNA gene sequences (cMAG 2016 and cMAG 2018) (details are provided in Supplementary Discussion), as well as ‘Ca. A. ciliaticola’ Sanger sequences retrieved from a clone library (clones 2, 6, 9, 13 and 15; details are provided in Methods), and amplified from individual (S1 and S3) or combined ciliates (C5 and C10), were included in the tree. ‘Candidatus Azoamicus’ subgroup A and B are highlighted in red. Four sequences belonging to the Alphaproteobacteria served as outgroup (Rickettsia rickettsia, L36217; Magnetospira thiophila, AB021370; Hyphomicrobium methylovorum, AB680579; and Rhodobacter blasticus, D16429). Bootstrap values >70% (out of 100 resamplings) are shown in front of the respective nodes, and the scale bar indicates nucleotide substitutions per site.
