Extended Data Fig. 1: Live imaging of large-scale organelle degradation in the lens of zebrafish.
From: Organelle degradation in the lens by PLAAT phospholipases
a, Live imaging of ER–eGFP and mito–mRFP in the lens of zebrafish. The equatorial diameter of the whole lens (black) and the mitochondria- (red) or endoplasmic-reticulum-free zone (green) was quantified in zebrafish (n = 16) at the indicated time points. Solid horizontal bars indicate medians, boxes the interquartile range (25th–75th percentiles) and whiskers 1.5× the interquartile range; outliers are plotted individually. Scale bar, 20 μm. b, Live imaging of ER–eGFP and mito–mRFP in the lens of zebrafish from 56 hpf (n = 3). Magnified time lapse-images in the indicated regions are shown in the right panels. A model of degradation of the endoplasmic reticulum and mitochondria is shown below. Scale bars, 20 μm (leftmost panels), 5 μm (all other panels). c, d, Live imaging of mito–mRFP (c) and ER–eGFP (d) in the lens of rb1cc1+/− (n = 7) and rb1cc1−/− zebrafish (n = 5). Scale bars, 20 μm.
