Extended Data Fig. 5: Progressive ensheathment of motor synapses by astrocytes across critical period closure. | Nature

Extended Data Fig. 5: Progressive ensheathment of motor synapses by astrocytes across critical period closure.

From: Astrocytes close a motor circuit critical period

Extended Data Fig. 5: Progressive ensheathment of motor synapses by astrocytes across critical period closure.

a–e, Time course of astrocyte–motor neuron synapse association from embryonic stage 17 (a), 0 h ALH (b), 4 h ALH (c), 8 h ALH (d) and 22 h ALH (e). Astrocytes, cyan (Gat+). Motor neuron membranes, green (myr::GFP+). Motor neuron post-synapses, magenta (mStrawberry+). a′–e′, Astrocytes and post-synapses alone. Synapses ≤ 90 nm from astrocyte membranes were counted as ensheathed. Scale bar, 5 μm. Genotype: RN2-gal4, UAS-myr::GFP, UAS-drep-2::mStrawberry. f, Quantification of astrocyte-associated post-synapses (percentage of total) revealed a significant interaction between developmental stage and per cent ensheathment (two-way ANOVA, P < 0.0001). N = 6 brains per time point, per cent ensheathment averaged over N ≥ 2 segments (A1–A2). Data are mean ± s.d. ****P < 0.0001. N values reflect biological replicates from 2 independent experiments.

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