Extended Data Fig. 6: Intra-subunit conformational changes of the TMD of GB2 upon activation.
From: Structural basis of GABAB receptor–Gi protein coupling
a, Overlay of the structures of the TMD of GB2 in antagonist-bound (antago) (PDB 7C7S) (yellow), agonist-bound (ago) (PDB 6UO9) (sky blue), and agonist- and PAM–Gi-bound (ago/PAM-Gi) (green) states. b, c, Overlay of the different states of the TM6 of GB1 (antago, grey; ago/PAM–Gi, red) (b) and the TM6 of GB2 (antago, yellow; ago, blue; ago/PAM–Gi, green) (c). d, e, NanoBiT G-protein dissociation assay of GABAB with alterations of the residues that are involved in activation. Data are mean ± s.e.m. from at least three independent experiments, performed in technical triplicate.
