Extended Data Fig. 9: Whole-cell recordings of projection neurons in response to correlated versus anti-correlated odour stimulation.
From: Fast odour dynamics are encoded in the olfactory system and guide behaviour
a, Schematic of the whole-cell patch-clamp recording approach. b, c, Distributions of input resistance (b) and recording depth (c) as measured from all recorded projection neurons (n = 31). d, Left, example recordings from single cells with consecutive presentations of correlated (black) and anti-correlated (red) odour stimulus at 2 Hz. Duration of odour presentation (2 s) is indicated in light blue. Right, baseline-subtracted and spike-clipped subthreshold voltage response from a single cell to odour 1 (green) and odour 2 (blue) for 2 Hz. e, As in d but for 20 Hz odour stimulation. f, Voltage response from three example cells for correlated (black) and anti-correlated (red) odour stimuli for 2 Hz (top) and 20 Hz (bottom). The cell shown in fi corresponds to the cell shown in d, e. The grey overlaid traces correspond to the arithmetic sum estimated from the response to individual odours. Bottom, linear prediction histogram calculated by thresholding the arithmetic sum of the subthreshold responses to the individual odours. Differences here suggest that correlation can be calculated at the single-cell level if the two individual odours engage overlapping OSN populations. P values are derived from a paired two-sided t-test of the membrane potential and the firing rate in the first 500 ms after odour onset. g, h, Average change in voltage (gi) and in instantaneous spike frequency (gii) in the first 500 ms after odour onset from baseline membrane potential for 2 Hz (g) and 20 Hz (h) correlated versus anti-correlated odour presentation. Each marker corresponds to a single cell; error bars represent s.e.m. Data points in black represent cells where P < 0.05 between correlated and anti-correlated conditions. P values are derived from a paired t-test of the membrane potential and the firing rate in the first 500 ms after odour onset. Indicators i, ii and iii represent cells shown in f. i, Pie charts depicting the proportions of cells showing significant difference as described above (blue) in subthreshold membrane potential (left) and spike frequency (right) for all 2 Hz (top) and 20 Hz (bottom) cells. P values are derived from a paired t-test of the membrane potential and the firing rate in the first 500 ms after odour onset.
