Extended Data Fig. 5: Effect of Alpl silencing on cellular respiration.
From: Mitochondrial TNAP controls thermogenesis by hydrolysis of phosphocreatine
a, Quantitative PCR with reverse transcription (qRT–PCR) of differentiated primary brown preadipocytes treated with shLacZ or shAlpl; n = 3 biologically independent samples per group. b, Western-blot analysis on TNAP in cells treated with shLacZ or shAlpl. Vinculin (VCL) blot was used as a sample processing control. c, Effect of Alpl knockdown (adenoviral shAlpl) on the oxygen consumption rate (OCR) of primary brown adipocytes. Treatments to initiate different respiration states are as follows: stimulated, noradrenaline; uncoupled, oligomycin; maximum, carbonyl cyanide m-chlorophenyl hydrazone; n = 11 biologically independent samples for the shLacZ group and 18 biologically independent samples for the shAlpl group. Data are presented as mean ± s.e.m. Statistical significance was calculated by unpaired Student’s two-sided t-test.
