Extended Data Fig. 11: Role of tuft cells in macrophage infiltration in mouse viral pneumonia model.
a, Immunofluorescence staining for SCGB1A1 and DCLK1 of proximal (left) and distal (right) airway from wild-type (WT) mice at baseline. n = 3 mice per group. Arrow, DCLK1+ cell. Scale bar, 50 μm. b, As in a, but in wild-type (left) and Pou2f3−/− mice 14 days after infection with H1N1 (PR8). c, Quantification of tuft cells as percentage of DCLK1+ cells in Pou2f3−/− compared to wild-type mice. Mean ± s.d., t-test. b, c, n = 4 mice per group. d, Immunofluorescence staining for CD45 and CD64 of lung parenchyma from wild-type (left) and Pou2f3−/− (right) mice 14 days after infection with H1N1 (PR8). Arrows indicate CD45+CD64+ macrophages. Scale bar, 50 μm. e, Quantification (CD45+CD64+ cells among CD45+ cells) as percentage in Pou2f3−/− mice compared to wild-type mice 14 days after infection with H1N1. Mean ± s.d., t-test. d, e, n = 3 mice per group. f, Gating strategy to identify CD45+CD64+F4/80+ cells. g, Identification of CD64+F4/80+ cells (based on gating strategy in f) in wild-type (left) and Pou2f3−/− mice (right) 14 days after infection with H1N1. h, Quantification of flow-cytometric determination of CD45+CD64+F4/80+ cells as percentage of CD45+ cells in Pou2f3−/− relative to wild-type mice (n = 3 per group). Mean ± s.d., t-test. i, qRT–PCR comparing relative mRNA expression of indicated chemokines and cytokines in Pou2f3−/− and wild-type mice 14 days after infection with H1N1 (n = 3 per group). Mean ± s.d., t-test. j, As in i, but 44 days after infection with H1N1 (n = 3 per group). k, Exemplary immunofluorescence staining (n = 3 mice per group) for KRT5 and DCLK1 in wild-type mouse 90 days after infection. Arrows indicate DCLK1+ cells. Scale bar, 50 μm. l, As in i, j, but comparing expression of indicated chemokines and cytokines in control donors and patients with COVID-19 (n = 3 donors per group). Mean ± s.d., t-test.
