Extended Data Fig. 8: Histology for chemogenetic manipulations of distinct ITC clusters.
From: Intercalated amygdala clusters orchestrate a switch in fear state
a, Examples of histological validation of KORD–mCitrine expression in ITCvm neurons across multiple anterior-to-posterior coronal sections. Following all the behavioural experiments (Fig. 3), mice were killed and slices (50 μm) cut and stained with an anti-FOXP2 antibody. Scale bar, 200 μm. b, Examples of histological validation of hM3Dq–mCherry expression in ITCvm neurons across multiple anterior-to-posterior coronal sections. Scale bar, 200 μm. c, Heatmaps illustrating virus expression aggregated across Cre+ mice in a and b. Scale bar indicates the fraction of animals exhibiting expression at each locus (0: no mice expressed; 1: all mice expressed). d, Examples of histological validation of KORD–mCitrine expression in ITCdm neurons across multiple anterior-to-posterior coronal sections. Scale bar, 200 μm. e, Examples of histological validation of hM3Dq–mCherry expression in ITCdm neurons across multiple anterior-to-posterior coronal sections. Scale bar, 200 μm. f, Heatmaps illustrating virus expression aggregated across Cre+ mice in d and e. Scale bar indicates the fraction of animals exhibiting expression at each locus (0: no mice expressed; 1: all mice expressed). Bregma levels are noted above the panels in a and d.
