Extended Data Fig. 1: Obesity accelerates hair loss with repeated hair cycle induction.
From: Obesity accelerates hair thinning by stem cell-centric converging mechanisms
a, Experimental design for a, f, g, and representative images of mice fed an ND or an HFD as indicated (n = 4). b, Experimental design for c–e, h. c–e, Representative images of genetically obese mice fed an ND with hair cycle induction at the indicated times (n = 4 mice each for control and obese). f, Representative images of C57BL/6J mice fed an ND or an HFD (n = 3). g, Representative images of female mice fed an ND or an HFD (n = 4). h–j, Representative images (h), fasting blood glucose levels (i) and body weights (j) of streptozocin-induced diabetic mice (ND, n = 4; HFD, n = 4; STZ-treated mice, n = 8; two-tailed Dunnett’s test, **P < 0.05; for exact P values see Source Data). k, Representative images of mice fed an ND or a high-sucrose diet (HSD; n = 4). l, Representative cross-section images of hair follicles from three ND-fed and three HFD-fed mice. Bulge and basal layer stained with COL17A1 and K14, respectively. m–o, Whole-mount images and bulge numbers for ob/ob mice (n = 3, two-tailed unpaired t-test) or HFD-fed female mice (n = 4, two-tailed unpaired t-test). Scale bars, 60 μm. p, Correlation between the degree of obesity and hair follicle number or the number of hair follicles without a bulge (sebaceous gland only) after five months’ treatment with HFD or ND with monthly hair depilation by plucking. Two-tailed Pearson’s correlation coefficient. ns, not significant; wo, without. Data shown as mean ± s.d.
