Extended Data Fig. 4: SHH signalling is inhibited specifically in HFSCs from HFD-fed mice.
From: Obesity accelerates hair thinning by stem cell-centric converging mechanisms
a, Col17a1 mRNA expression in telogen HFSCs from young, old, ND-fed and HFD-fed mice without depilation (3-month feeding, n = 3, two-tailed unpaired t-test) derived from microarray data. b, Motif analysis of ATAC–seq data with binomial P values. TF, transcription factor. c–e, RNA-seq analysis of anagen HFSCs from 6-month-old HFD-fed (4 months) and ND-fed mice (n = 2). c, Pathways downregulated in anagen HFSCs from HFD-fed mice were analysed with GSEA by choosing c2.cp.biocarta.v7.2 (curated gene sets) as the gene set correlation. All pathways with GSEA P < 0.05 are shown. d, Enrichment plot for the SHH pathway Biocarta gene set. e, Heatmap of the Biocarta SHH pathway gene set core signature (red to dark blue, high to low expression in the space of the analysed gene set). f, qPCR analysis for Gli1and Ptch1 of HFSCs from 7-week-old ob/ob and db/db mice (n = 4, two-tailed unpaired t-test). ctrl, control t, telogen; a, anagen. Data shown as mean ± s.d.
