Extended Data Table 3 IP accumulation assays of wild-type and mutant mGlus using a chimeric Gα protein Gαqi9

From: Structures of human mGlu2 and mGlu7 homo- and heterodimers

  1. †The basal activity was calculated by subtracting the IP production measured in the control (Gαqi9) for the wild-type receptor and all the mutants, and is presented as per cent of wild-type activity.
  2. ‡Data are mean ± s.e.m. from at least three independent experiments. nd, not determined (data for which a robust concentration response curve could not be established within the concentration range tested). ***P < 0.001 by one-way ANOVA followed by Dunnett’s post-test compared to the response of wild type.
  3. §Sample size, the number of independent experiments performed in technical triplicate.
  4. ||Protein expression levels of mGlu2 and mGlu7 constructs at the cell surface were determined in parallel by flow cytometry with an anti-Flag antibody (Sigma) and reported as per cent compared to the wild type from at least three independent measurements performed in duplicate.
  5. The mutations were introduced in the wild-type receptor.
  6. #The span is defined as the window between the maximal agonist response (Emax) and the vehicle (no agonist or PAM).
  7. ††The half-maximal inhibitory concentration (IC50) ratio (IC50(mutant)/IC50(WT)) characterizes the effect of mutations on inhibitory activity of NAMs.
  8. ‡‡n = 29.
Back to article page