Fig. 2: SARS-CoV-2 infection elicits S-binding long-lived BMPCs.
From: SARS-CoV-2 infection induces long-lived bone marrow plasma cells in humans
a, Representative images of ELISpot wells coated with the indicated antigens or anti-immunoglobulin (Ig) and developed in blue and red for IgG and IgA, respectively, after incubation of magnetically enriched BMPCs from control individuals and convalescent individuals. b, Frequencies of BMPCs secreting IgG (left) or IgA (right) antibodies specific for the indicated antigens, indicated as percentages of total IgG- or IgA-secreting BMPCs in control individuals (black circles) or convalescent individuals 7 months (white circles) or 11 months (grey circles) after symptom onset. Horizontal lines indicate the median. P values from two-sided Kruskal–Wallis tests with Dunn’s correction for multiple comparisons between control individuals and convalescent individuals. Each symbol represents one sample (n = 18 convalescent, n = 11 control). c, Paired frequencies of S-binding BMPCs among IgG-secreting (left) and IgA-secreting (right) BMPCs from convalescent individuals 7 months and 11 months after symptom onset. d, Paired anti-S (left) and anti-RBD (right) IgG serum antibody titres from convalescent individuals 7 months and 11 months after symptom onset. Data in c and d (left) are also shown in b and Fig. 1b, respectively. Each symbol represents one sample (n = 5). Dotted lines indicate the limit of detection. e, Frequencies of BMPCs secreting IgG antibodies specific for SARS-CoV-2 S (left) and influenza virus vaccine (right) plotted against respective IgG titres in paired blood samples from control individuals (black circles) or convalescent individuals 7 months after symptom onset (white circles). P and r values from two-sided Spearman’s correlations. Each symbol represents one sample (n = 18 convalescent, n = 11 control).
