Extended Data Fig. 4: Y396 and Y406 are the main, if not sole, tyrosine residues in DOK7 that are phosphorylated by AGRIN stimulation. | Nature

Extended Data Fig. 4: Y396 and Y406 are the main, if not sole, tyrosine residues in DOK7 that are phosphorylated by AGRIN stimulation.

From: Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia

Extended Data Fig. 4

We generated muscle cell lines from wild-type and Dok72YF/2YF mice and treated the cultured myotubes with AGRIN for 30 min. MUSK was immunoprecipitated, and western blots were probed with antibodies against MUSK or phosphotyrosine (pTyr). AGRIN stimulates DOK7 tyrosine phosphorylation in wild-type but not Dok72YF/2YF myotubes (data from two experiments).

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