Extended Data Fig. 5: MUSK antibody clones.
From: Mechanism of disease and therapeutic rescue of Dok7 congenital myasthenia
a, Amino acid sequences of the complementarity-determining regions (CDRs) of antibodies against MUSK developed in this study. CDR definitions are based on Wu and Kabat38, except that CDR-H1 includes four additional residues at the N terminus to show diversified positions. b, Amino acid sequences of the VL and VH domains of clone X17. c, d, Binding titration of antibodies against MUSK in the Fab format to immobilized hFz, hECD, mFz and mECD, as tested using a bead-based binding assay. Curves show the best fit of the 1:1 binding model. The table lists apparent KD values (mean ± s.d., n = 3). The datasets in c and d were obtained different instruments, which resulted in different signal ranges. e, Binding titration of antibodies against MUSK in the IgG format, as in c.
