Extended Data Fig. 8: Antibody blocking of interactions between ACE2 and the 19 RBD mutants, the frequency of RBD mutations and mutational effects on RBD functionality.
From: Nasal delivery of an IgM offers broad protection from SARS-CoV-2 variants
a–s, The dose-dependent blocking of the interactions between ACE2 and the indicated RBD mutants. t, The correlation between ACE2 blocking IC50 and neutralization NT50 values. Two-tailed Spearman correlation was used in the statistical analysis. u, The frequency of SARS-CoV-2 circulating variants with indicated RBD mutations. The Tracking Mutations tool was used for analysis as described in the methods. v, The effects of indicated RBD mutations on RBD binding affinity to ACE2 and RBD protein expression. The online web source on the sequence-to-phenotype maps of the RBD of SARS-CoV-2 (https://jbloomlab.github.io/SARS-CoV-2-RBD_DMS/) was referred to for analysis. The y axis indicates log10 scale changes to wild-type RBD. Positive values indicate an improving effect and negative values indicate a decreasing effect by the mutations.
