Extended Data Fig. 6: Isolated-B1 Events of HCN1SM.
From: cAMP binding to closed pacemaker ion channels is non-cooperative
a, Dwell time distributions of isolated-B1 events for HCN1SM at various fcAMP concentrations overlaid with maximum likelihood estimates for monoexponential (blue dashed) and biexponential (red) distributions (Supplementary Table 3). For inset, error bars are the error of a binomial distribution (Methods). b, c, Coordinates of identified single-molecules in the 512 × 512 pixel field of view superimposed across all ZMW arrays. The colour bars denote the average dwell time (b) and fluorescence (c) of the isolated-B1 state for each molecule (n). d, Correlation of fluorescence intensity and dwell times for each isolated-B1 event (m), where r is the Pearson correlation coefficient. Data are binned for visualization.
