Extended Data Fig. 8: Isolated-B1 events do no exhibit static heterogeneity.
From: cAMP binding to closed pacemaker ion channels is non-cooperative
a, e, Average isolated-B1 dwell time of HCN1SM (a) and HCN2SM (e) for each molecule at 100 nM fcAMP. Outliers (diamonds) were identified by three scaled median absolute deviations. Data plotted as mean ± s.d. of exponential distribution. The blue dashed line indicates the average B1 dwell time across all molecules (HCN1SM: n = 176; HCN2SM: n = 77). b, f, Histograms of average isolated-B1 dwell times for each HCN1SM (b) and HCN2SM (f) molecule. c, g, Parameters for a monoexponential fit (τ) to isolated-B1 dwell times of HCN1SM (c) and HCN2SM (g). d, h, Parameters for a biexponential fit (τ1, τ2, A1) to isolated-B1 dwell times of HCN1SM (d) and HCN2SM (h). For c, g, d, h, the ordinate corresponds to the obtained parameter (τ, τ1, τ2, A1) and error bars are 95% confidence intervals. All parameters were obtained using maximum likelihood estimates across all isolated-B1 events in either all data (HCN1SM: n = 8,229; HCN2SM: n = 2,676) or inlier (HCN1SM: n = 7,816; HCN2SM: n = 2,575) groups, as indicated on the abscissa.
