Extended Data Fig. 1: Cryo-EM analysis of the human Cav2.2 complex alone and in the presence of ziconotide.
From: Structure of human Cav2.2 channel blocked by the painkiller ziconotide
a, Representative electron micrograph and 30 classes of 2D class averages for Cav2.2–ziconotide. The green circles indicate particles in distinct orientations. The box size for the 2D averages is 312 Å. Scale bar, 50 nm. Left, a half view of one micrograph out of 3,384 in total for Cav2.2–ziconotide. b, Workflow for electron microscopy data processing (Methods). c, The gold-standard Fourier shell correlation (FSC) curves for the 3D reconstructions. The graph was prepared in GraphPad Prism. d, FSC curves of the refined model versus the summed map that it was refined against (black); of the model versus the first half map (red); and of the model versus the second half map (green). Z-complex, Cav2.2–ziconotide.
