Table 1 Key amino acids in PF4 for the binding of VITT and HIT antibodies
From: Antibody epitopes in vaccine-induced immune thrombotic thrombocytopaenia
Critical PF4 amino acids for VITT antibodies | |||
|---|---|---|---|
PF4 mutant | Mean loss of binding compared to wild-type PF4 (%) | Range (%) | Number of VITT samples affected |
R22A | 79.9 | 51.8–91.7 | 5/5 |
H23A | 68.0 | 26.7–77.5 | 3/5 |
E28A | 68.5 | 48.4–88.1 | 5/5 |
K46A | 72.6 | 17.5–84.2 | 4/5 |
N47A | 54.3 | 31.6–66.4 | 5/5 |
K50A | 82.2 | 11.6–90.5 | 4/5 |
K62A | 58.8 | 15.3–77.3 | 3/5 |
K66A | 54.1 | 2.2–88.8 | 4/5 |
Critical PF4 amino acids for HIT antibodies | |||
PF4 mutant | Mean loss of binding compared to wild-type PF4 (%) | Range (%) | Number of HIT samples affected |
L8A | 48.6 | 12.4–76.0 | 6/10 |
C10A | 49.4 | 5.0–73.3 | 6/10 |
C12A | 45.7 | 4.8–74.7 | 7/10 |
T16A | 41.1 | 4.0–78.4 | 6/10 |
R22A | 36.2 | 1.3–63.0 | 5/10 |
Q40A | 35.5 | 2.2–75.5 | 3/10 |
N47A | 31.4 | 3.9–80.1 | 3/10 |
C52A | 55.8 | 25.0–73.3 | 8/10 |
L53A | 51.3 | 36.1–74.8 | 5/10 |
D54A | 47.1 | 0.8–76.0 | 6/10 |
K61A | 47.4 | 1.7–82.2 | 6/10 |
K66A | 40.4 | 5.1–89.6 | 5/10 |
L67A | 48.5 | 6.4–85.8 | 5/10 |
