Extended Data Fig. 10: Inhibiting lipid export or lactate intake in projection neurons partially rescues the decline of olfaction sensitivity upon infection and during ageing.
From: Gut cytokines modulate olfaction through metabolic reprogramming of glia
a, Preference index of young infected flies after knockdown of dome, stat, GLaz or out in the EG with additional RNAi lines. b, c, Intake of total food, Ecc15+ food and normal food for mock flies during homeostasis and for infected flies after the loss of dome, stat, GLaz or out, measured by CAFE assay. The GLazRNAi and outRNAi lines in b and c are different. d, Survival curve of young flies after loss of GLaz or out upon continuous PE infection. The GLazRNAi and outRNAi lines are as in b. e, Representative images showing LD accumulation and lipid peroxidation at the AL from old flies after knockdown of GLaz or out in EG driven by GMR56F03::Gal4;tubG80ts. LD numbers per AL were quantified. Lipid peroxidation levels of LDs for each sample were measured as the mean 488/561 nm intensity ratio. The ratios were normalized to the mean value of old control samples. Flies were aged at 25 °C for 14 days followed by 29 °C for 14 days to induce expression of RNAi lines. f, Preference index values of old flies overexpressing Lip-4, or knockdown of NLaz, Ldh or out in projection neurons using the GH146::Gal4 driver. g, Intake of total food, Ecc15+ food and normal food for young infected flies after knockdown of NLaz or out in projection neurons. h, Survival curve of flies after knockdown of NLaz or out in projection neurons upon continuous PE infection. i, Intake of total food and normal food for young infected flies overexpressing Lip-4 in projection neurons. Data are mean and s.e.m. The sample size is as follows: n = 5, 5, 5, 5, 5, 5 and 4 independently performed experiments per condition (from left to right) in a, n = 6, 6, 5 and 7 replicates (3 flies per cohort) for mock mCherryRNAi, Ecc15 mCherryRNAi, Ecc15 GLazRNAi and Ecc15 outRNAi correspondingly in b, n = 8, 8, 8, 7 and 6 replicates (3 flies per cohort) for mCherryRNAi, domeRNAi, statRNAi, GLazRNAi and outRNAi correspondingly in c, n = 100, 59 and 85 flies for mCherryRNAi, GLazRNAi and outRNAi respectively in d, n = 15, 7 and 7 brains per condition (from left to right) in e, n = 13, 8, 7, 8 and 7 independently performed experiments per condition (from left to right) in f, n = 6, 7, 8 and 7 replicates for mCherryRNAi, NLazRNAi, outRNAi(v51157) and outRNAi(BL67858) correspondingly in g, n = 49, 86, 87 and 53 flies for mCherryRNAi, NLazRNAi, outRNAi(v51157) and outRNAi(BL67858), respectively, in h, n = 8 replicates (3 flies per cohort) per condition in i. Data in b–d, g and h are representative of two independently performed experiments, and those shown in e and i are representative of three separate experiments. P values in d and h from log-rank test; P values in a (when comparing mCherryRNAi, LacZRNAi and UAS::LacZ), c (top: when comparing mCherryRNAi, domeRNAi and GLazRNAi; bottom) and g from Kruskal–Wallis test; other P values from two-tailed Mann–Whitney test.
