Extended Data Fig. 3: JAK–STAT signalling in EG promotes avoidance behaviour against Ecc15, yet increasing host survival upon acute infection.

a, Preference index of young flies with indicated JAK–STAT perturbation in the EG. RNAi constructs were expressed in the EG for 7 days by shifting flies to 29 °C (restrictive temperature for Gal80^ts). Flies were exposed to Ecc15 for 24 h. b, Preference index of young infected flies expressing mCherry^RNAi, dome^RNAi or stat^RNAi in all glia (repo::Gal4;tubG80^ts), measured by T-maze assay. c, Quantification of STAT::GFP activity in the glia of flies after dome^RNAi or stat^RNAi knockdown in all glia (repo::Gal4; 10xSTAT::GFP), to confirm knockdown efficiency for various RNAi lines that target Dome or STAT, respectively. d, e, Total food intake and normal food intake of flies overexpressing hop^tuml (d) and of infected flies after knockdown of dome and stat (e) in EG (driven by GMR56F03::Gal4;tubG80^ts), measured by CAFE assay. f, Intake of total food, Ecc15-containing food and normal food for flies expressing mCherry^RNAi, LacZ^RNAi and UAS::LacZ in the EG during homeostasis. g, h, Survival curve of flies overexpressing hop^tuml (g) or knocking down dome (h) in the EG after continuous PE infection. i, Survival curves of wild-type flies (w¹¹¹⁸ × OreR), Gr63a¹ flies, Orco¹ flies after continuous PE infection. Data are mean and s.e.m. The sample size is as follows: n = 6, 7, 5, 5 and 5 independently performed experiments per condition (from left to right) in a, n = 4 independently performed experiments per condition in b, n = 7, 8, 8, 6 and 7 brains per condition (from left to right) in c, n = 8 and 9 replicates (3 flies per cohort) for mCherry^RNAi and hop^tuml, respectively in d, n = 6, 8 and 7 replicates (3 flies per cohort) for mCherry^RNAi, dome^RNAi and stat^RNAi in e, n = 8 replicates per condition (3 flies per cohort) for f, n = 74 and 96 flies for mCherry^RNAi and hop^tuml, respectively, for g, n = 97 and 118 flies for mCherry^RNAi and dome^RNAi, respectively, for h, n = 101, 63 and 90 flies for wild-type flies (w¹¹¹⁸ × OreR), Orco¹, Gr63a¹ flies for i. Data in d, e, g, h are representative of three independently performed experiments; data shown in c, f and i are representative of two independently performed experiments. P values from two-tailed Mann–Whitney test in a (mCherry^RNAi compared with hop^tuml), c, d; P values from log-rank test in g–i; other P values from Kruskal–Wallis test.

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