Fig. 3: Increased IFB-2 levels induce age-related intestinal alterations.
From: Rewiring of the ubiquitinated proteome determines ageing in C. elegans
a, Intestinal-specific knockdown (KD) of ifb-2 extends lifespan. P value determined by two-sided log-rank test; n = 96 worms per condition. Lifespan data are representative of two independent experiments. Supplementary Table 11 contains replicate data of independent experiments. b, Filter trap analysis with an antibody against IFB-2 of wild-type worms at different ages. Representative of eight independent experiments. c, Filter trap analysis with an antibody against IFB-2 of wild-type and IFB-2 (Ub-less) mutant worms. Representative of four independent experiments. d, e, Filter trap experiments with an antibody against GFP of worms expressing IFC-1::GFP under the ifc-1 promoter (d) or IFP-1::GFP under the ifp-1 promoter (e). Representative of two independent experiments. f, Quantification of bacterial colonization. Fluorescence of mCherry-expressing E. coli within the intestine relative to day 1 (D1) Vector RNAi. Data are mean ± s.e.m. D1 Vector RNAi, n = 56 worms from 3 independent experiments; D1 ifb-2 RNAi, n = 35; D5 Vector RNAi, n = 53; D5 ifb-2 RNAi, n = 55; D10 Vector RNAi, n = 45; D10 ifb-2 RNAi, n = 39. g, Bacterial colonization relative to day-10 adult wild-type worms. Data are mean ± s.e.m. Wild-type, n = 50 worms from 3 independent experiments; Ub-less IFB-2, n = 47. In f and g, P values were determined by two-sided t-test. NS, not significant. In all experiments, RNAi was initiated at day 1 of adulthood.
