Fig. 4: Increased EPS-8 levels shorten lifespan through RAC hyperactivation.
From: Rewiring of the ubiquitinated proteome determines ageing in C. elegans
a, b, Muscle-specific (a) and neuronal-specific (b) knockdown of eps-8 after development extends lifespan. c, Knockdown of mig-2 after development extends longevity and rescues the short lifespan induced by ubiquitin-less EPS-8. P values in a–c were determined by two-sided log-rank test; n = 96 worms per condition. Supplementary Table 11 contains replicate data of independent experiments. d, Staining of filamentous actin with phalloidin. eps-8 RNAi prevents age-associated destabilization of actin filaments. Scale bar, 20 μm. Representative of two independent experiments. e, Knockdown of mig-2 rescues the disruption of actin filaments in day-3 adult worms induced by ubiquitin-less EPS-8. Scale bar, 20 μm. Representative of two independent experiments. f, Thrashing movements per 30 s (n = 45 worms per condition from three independent experiments). Knockdown of mig-2 suppresses motility deficits induced by ubiquitin-less EPS-8 in young adult worms. Data are mean ± s.e.m. P values in f determined by two-sided t-test. In all experiments, RNAi was initiated at day 1 of adulthood.
