Extended Data Fig. 9: Cryo-EM reconstruction suggests that the previously claimed ‘PilA-N-alone’ filament could be DNA. | Nature

Extended Data Fig. 9: Cryo-EM reconstruction suggests that the previously claimed ‘PilA-N-alone’ filament could be DNA.

From: Structure of Geobacter pili reveals secretory rather than nanowire behaviour

Extended Data Fig. 9

a, Cryo-EM image of filaments claimed to be PilA-N alone3. b, Cryo-EM images showed similar filaments in our OmcS filament preparations. Scale bars, 20 nm (a, b). Black arrows represent OmcS filaments in a, b. c, Two-dimensional average showed no helical features consistent with T4P. Scale bar, 5 nm. d, e, Cryo-EM density map (d) and docking with DNA molecule (e) (PDB code 1BNA), suggested the identity of the filaments to be DNA.

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