Extended Data Fig. 10: Expression of PilA-N–C filaments restores the secretion of OmcS and OmcZ nanowires in ΔpilA-N cells.
From: Structure of Geobacter pili reveals secretory rather than nanowire behaviour
a, AFM images of ΔpilA-N/pilA-N-C cell showed pili on the surface of the cell. b, Zoomed-in image of ΔpilA-N/pilA-N-C cell. c, Height analysis of the pili consistent with PilA-N–C filament. d, e, ΔpilA-N/pilA-N-C cell showed the secretion of OmcS (d) and OmcZ (d) nanowires. Scale bars, 1 μm (a), 300 nm (b), 100 nm (d, e). f, Height analysis of filaments at locations shown in e showed the diameter consistent with OmcS and OmcZ filaments. g, Immunoblotting with OmcS antibody showing the restoration of secretion defect in OmcS nanowires in ΔpilA-N/pilA-N-C cells. FP, filament preparation; PP, periplasmic fraction; M, marker. For gel source data, see Supplementary Fig. 16. h, i, TEM image of filament preparation from ΔpilA-N showing no filament (h) and OmcS filaments from ΔpilA-N/pilA-N-C (i). Scale bars, 200 nm (h, i). ΔpilA-N/pilA-N-C: in-trans expression of an episomal copy of wild-type pilA-N and pilA-C in ΔpilA-N.
