Extended Data Fig. 6: Post-translational modifications in PilA-N–C filament.
From: Structure of Geobacter pili reveals secretory rather than nanowire behaviour
a, Lack of PTM in Y32 of PilA-N. Mass spectra of PilA-N did not show any modified peptide in AYNSAASSDLR with expected mass of 1.154 kDa. Inset, MS/MS spectra showed no modification on Y32 in PilA-N. b, Mass spectra showed methylated peptide FTLIELLVVAIIGILAAIAIPQQFSAYR with mass 3.044 kDa. Inset, MS/MS spectra showed N-terminal methylation. c, Cryo-EM map for PilA-C showing an extra density on Ser94, suggesting a post-translational modification. d, Gel of purified filaments showing glycosylated PilA-C (left lane) and positive control using horseradish peroxidase (right lane).
