Extended Data Fig. 9: Proliferation and differentiation potential of HPV-specific stem-like CD8 T cells. | Nature

Extended Data Fig. 9: Proliferation and differentiation potential of HPV-specific stem-like CD8 T cells.

From: Functional HPV-specific PD-1+ stem-like CD8 T cells in head and neck cancer

Extended Data Fig. 9

a, Flow plots showing the gating strategy to isolate stem-like (CD39−Tim-3−) and terminally differentiated cells (CD39+Tim-3+) PD-1+CD45RA− CD8 T cells. Histograms showing TCF-1 expression to validate that the sorting strategy using CD39 and Tim-3 as surrogate markers enriches for stem-like (green) and terminally differentiated (blue) cells. b, Representative plots showing CTV dilution and expression of CD45RO, CD25 and CD28 after five days of culturing stem-like and terminally differentiated CD8 T cells alone or with peptide-pulsed PBMCs. Summary plots show percentage of cells positive for the indicated markers on day five. Means and their SD are represented, ** < 0.01, ns = not significant, unpaired Mann-Whitney U test.

Back to article page