Extended Data Fig. 2: 18O2 labelling of human cells reveals the oxy-metabolome.
From: The polar oxy-metabolome reveals the 4-hydroxymandelate CoQ10 synthesis pathway
a, Schematic of the approach used to identify 18O-labelled features and metabolites that were labelled by 18O. n represents the number of features or metabolites identified in MIAPACA2 cells grown at 3% 18O2. See b for details. b, Summary of total and percentage of identified 18O-labelled features for each cell line and oxygen tension as described in a. c, Venn diagram demonstrating the overlap of unique 18O-labelled metabolite and features identified for each oxygen condition per cell line. d, Total number of unique dioxygenase-dependent, 18O-labelled metabolites and features identified in each cell line and condition. Features were categorized into predicted or not predicted/unknown 18O-labelled metabolites, based on known oxygen-dependent metabolic pathways, and sensitivity or insensitivity to IOX1 (dioxygenase inhibitor) treatment. e, Number of 18O-labelled metabolites detected in cells grown in 3%, 1%, and 0.2% 18O2 for 24 h in two sets of experiments. The overlap of the total number of detected 18O-labelled metabolites and features in both experimental sets are shown. f, Venn diagram representing the distribution of common and unique 18O-labelled metabolites identified in each cell line. g, List of the 46 unique 18O-labelled metabolites that were identified in f and categorized into known oxygen-dependent metabolic pathways. ** represents metabolites that have matching MS2 spectra, but need to be validated due to multiple metabolite isomers.
