Extended Data Fig. 6: The expression of Pyrin and ZBP1 is not regulated by GSDMD or caspase-8, -7, -3 or -6 during HSV1 or F. novicida infections.
From: AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence
a, Immunoblot analysis of caspase-1 (CASP1) activation and ZBP1 and Pyrin expression in wild type (WT) or Gsdmd−/− bone marrow-derived macrophages (BMDMs) after HSV1 or F. novicida infection. Data are representative of at least three independent experiments. b, c, Immunoblot analysis of ZBP1, Pyrin and AIM2 expression in the indicated BMDMs after HSV1 (b) or F. novicida (c) infections. Data are representative of at least three independent experiments. d, Immunoblot analysis of ZBP1, Pyrin and AIM2 expression in the indicated BMDMs after influenza A virus (IAV) infection. Data are representative of at least three independent experiments. For gel source data, see Supplementary Figure 1.
