Extended Data Fig. 7: Data collection and processing of the S/S2X58 complex cryoEM datasets.
From: Lectins enhance SARS-CoV-2 infection and influence neutralizing antibodies
a-b, Representative electron micrograph and 2D class averages of SARS-CoV-2 S in complex with the S2X58 Fab embedded in vitreous ice. Scale bar: 400 Å. c, Gold-standard Fourier shell correlation curves for the S2X58-bound SARS-CoV-2 S trimer in one RBD closed (black line) and three RBDs open conformations (gray line). The 0.143 cutoff is indicated by a horizontal dashed line. Due to steric clashes between the S2X58 Fab and the NTD of a neighboring monomer in the closed S state, this mAb appears to conformationally select the open RBDs, thus explaining its fusogenic activity. d, Local resolution maps calculated using cryoSPARC for the SARS-CoV-2 S/S2X58 Fab complex structure with one RBD closed and three RBDs open shown in two orthogonal orientations. e, Cryo-EM data collection statistics.
