Extended Data Fig. 2: Expression of attachment receptors in infected tissues.
From: Lectins enhance SARS-CoV-2 infection and influence neutralizing antibodies
a, Distribution and expression of ACE2, DC-SIGN, L-SIGN, and SIGLEC1 in the human lung cell atlas. b, Major cell types with detectable SARS-CoV-2 genome in bronchoalveolar lavage fluid and sputum of severe COVID-19 patients. Left panel shows a t-SNE embedding of single-cell gene expression profiles coloured by cell type and sized by viral load (logCPM); right panel, distribution plots by annotated cell type denote the cumulative fraction of cells (y-axis) with detected viral RNA per cell up to the corresponding logCPM value (x-axis). Viral RNA is also found in neutrophils, plasma and T cells – an observation that has been reported previously21,64,65,66,67,68,69,70 c, SIGLEC1 surface expression comparison. Mean number of SIGLEC1 antibody binding sites per cell displayed by SIGLEC1 stably transduced cell lines and different myeloid cells left untreated or exposed to IFNα or LPS for 48 h and assessed by quantitative FACS analysis. Data show mean values and SEM from one experiment including cells from 3 donors.
