Extended Data Fig. 7: Dot plot illustrating RNA expression levels (red) and hypo-CG-DMR motif enrichments (blue) of transcription factors (TFs) in mouse MOp subclasses.
From: A multimodal cell census and atlas of the mammalian primary motor cortex
The size and color of red dots indicate the proportion of expressing cells and the average expression level in each subclass, respectively. The size and color of blue dots indicate adjusted P-value (Fisher’s exact test, Benjamini-Hochberg Procedure) and log2(Odds Ratio) of motif enrichment analysis, respectively. Combining these two orthologous pieces of evidence identified many well-studied TFs in embryonic precursors, such as the Dlx family members for pan-inhibitory neurons, and Lhx6 and Mafb for MGE derived inhibitory neurons. We further identified many additional TFs with more restricted patterns in specific subclasses, such as Rfx3 and Rreb1 (in L2/3 IT), Atoh7 and Rorb (in L4/5 IT), Pou3 family members (in L5 ET), Etv1 (in L5/6 NP), Esrr family members (in Pvalb), and Arid5a (in Lamp5).
