Extended Data Fig. 14: Analysis of axonal inputs to the mPFC and dendritic spines in Rarb and Rxrg dKO mice.

Anteroposterior series of PD 30 coronal sections of representative WT (a) and dKO (b) with retrograde viral tracer AAVrg-Cag-Gfp injected into mPFC (green asterisk). Sections were inverted and converted to greyscale. Black regions indicate the presence of viral tracer. High magnification representative images of labelled regions (c) and quantification of labelling using 0-3 scale (d) (N = 4 for each genotype; see Methods section for more details). e, Quantification of Sholl analysis (Two-way ANOVA with Sidak’s multiple comparison method; P = .440- >0.999); Error bars: S.E.M.) and dendritic spines of upper layer neurons in the contralateral mPFC in WT (blue) and dKO (orange) (N = 4 for each genotype). Two-way ANOVA with Sidak’s multiple comparison method was applied; *P = 0.09; ****P < 1e-4; Error bars: S.E.M. Inset shows representative images of dendritic spines. Scale bar: 5 μm. ACAd, Dorsal anterior cingulate area; AI, Anterior Insula; BLA, Basolateral amygdala; vHPF, ventral hippocampal fields; CLA, claustrum; cl mPFC, Contralateral medial prefrontal cortex; ILA, Infralimbic area; LHA, Lateral habenula; MD, The mediodorsal nucleus of the thalamus; MOs/p, The secondary and primary motor areas. PER, Perirhinal cortex PIR, Piriform cortex PL, Prelimbic area; SSp, Primary somatosensory area; STN, Subthalamic nucleus; VTA, Ventral tegmental area.