Extended Data Fig. 2: Changes in internal states affects arousal threshold during sleep.
From: Sensory processing during sleep in Drosophila melanogaster

a, Schematic showing experimental procedures used in b, c and main Fig. 2a, b. b, Responses to 5% acetic acid in mock control flies (left) and flies previously intoxicated with vapours of 20% ethanol (right). Ethanol or mock exposure were limited to a 1-hour window between ZT15.5-16.5 and arousal was quantified and shown for ZT18-20. c, Quantification of the stimulus-evoked response in rested control flies (left) or flies that were previously deprived of sleep for 12h (right). Sleep deprivation was performed during the subjective night (ZT12-24) and waking to an olfactory stimulus (5% acetic acid) was measured during the subsequent day (ZT0-6). In all panels, errors are shown as bootstrapped 95% CI.