Extended Data Fig. 2: Antibody-binding characteristics of HA107-binding monoclonal dimeric IgA.
From: Parallelism of intestinal secretory IgA shapes functional microbial fitness
a, Cloning and recombinant antibody expression strategy for mouse dimeric IgA. b, c, f, Additional data showing further mIgAs that are not included in Fig. 1a–c due to space limitations. b, Individual monoclonal IgA binding to intact live HA107 bacteria by flow cytometry (top panels), or the HA107 lysate (bottom panels) by ELISA. Red dashed lines indicate cutoff for positivity. c, Flow cytometry analysis of mIgA binding of non-auxotrophic JM83 parental strain of HA107. d, Flow cytometric analysis showing distinctive nonreplicating, replicating and dying subpopulations of JM83 isolated from fresh faecal pellets of antibody-deficient mice ex vivo. e, Flow cytometric microscopy of faecal JM83 nonreplicating (top), replicating (middle) and dying (bottom) subpopulations stained with mIgA4250. f, Binding of individual monoclonal IgAs to faecal JM83 bacterial nonreplicating, replicating or dying subpopulations gated as in Extended Data Fig. 2d. mIgA clone numbers are indicated at the top of each column (b, c, f). Data are of one (e) or representative of two (b–d, f) independent experiments.
