Extended Data Fig. 3: Intestinal dimeric mIgAs bind discrete bacterial membrane fractions.
From: Parallelism of intestinal secretory IgA shapes functional microbial fitness
a–d, ELISA binding of individual monoclonal dimeric IgAs ordered as in Fig. 1a–c (a, b) or Extended Data Fig. 2b, c, f (c, d) to HA107 membrane fraction (top), cytoplasmic fraction (middle) and purified ribosomes (bottom) (a, c) or intact outer membrane vesicles (b, d). mIgA clone numbers are indicated at the top of each column. Red dashed lines indicate upper limit of binding from negative control antibody mIgAmGO53. The insets within the mIgA4559 (a, b) and the mIgA4025 (c, d) column show control serum IgG binding from a mouse injected with 108 c.f.u. of E. coli HA107 14 days previously.
