Extended Data Fig. 6: Mitochondria overmerging corrections.

a, Whole cell, 3D rendering of mitochondria in jrc_hela-2 segmented using naive connected component analysis method. Scale bar is 4 μm. b, FIB-SEM and raw mitochondria predictions thresholded at 127 (d = 0 nm) for the boxed region in (a), shown for one 2D slice. c, Naive connected component segmentation of mitochondria for the region in (a), performed on smoothed, thresholded predictions at 127 and followed by size filtering and hole-filling. d, To alleviate overmerging of mitochondria, we smooth the predictions and perform watershed segmentation on all voxels greater than or equal to 127. Shown are the resultant watershed fragments. e, To create the improved mitochondria segmentations, we agglomerate adjacent fragments in d based on parameters that best optimize the resultant segmentations, as chosen by an expert user. f, Final whole-cell rendering of corrected mitochondria predictions. Scale bar is 4 μm.