Extended Data Fig. 9: Analysis and biological insight.
From: Whole-cell organelle segmentation in volume electron microscopy
a, Relative volume occupied by each predicted organelle, per cell. MT volume only shown for jrc_hela-2. b, ER predictions in jrc_macrophage-2. Left panel is a 2D FIB-SEM slice with overlaid ER predictions in green, the middle panel shows a 3D rendering of the ER predictions, and the right panel shows the ER medial surface partitioned into planar and tubular structures and corresponding tubule thicknesses (colour bar). Scale bar is 500 nm. c, ER predictions in jrc_hela-2. Left panel is a 2D FIB-SEM slice with overlaid ER predictions in green and mitochondria predictions in orange (bottom), the middle panel shows a 3D rendering of the ER predictions and mitochondria predictions (bottom), and the right panel shows the ER medial surface partitioned into planes and tubes along with tubule thicknesses (colour bar). Also shown in the bottom right panel are the contact site regions (blue) where ER and mitochondria are within 10 nm of each other. Scale bars are 500 nm. d, Quantification of the peripheral ER curvature and surface area compared between jrc_hela-2 and jrc_macrophage-2. e, Quantification of the peripheral ER curvature at contact sites between peripheral ER and mitochondria, for jrc_hela-2 and jrc_macrophage-2.
