Extended Data Fig. 2: Comparison of NPC structures from purified NEs and cryo-FIB suggests the cellular environment impacts NPC architecture and IR diameter.
From: The cellular environment shapes the nuclear pore complex architecture
a, Comparison of human NPC density maps from cryo-FIB and nuclear envelope purification techniques. Measurements for the membrane-to-membrane and IR central channel diameters, as well as the height across the NE are shown. Interestingly, an asymmetric membrane curvature may also exist at human NPCs in HIV-infected cells22, though the angles of the membranes appear different than ours. We refrain from making any speculations about this asymmetry because the deposited map comes from cells infected with HIV-1 virus. b, Measurements of NPC diameter from single NPCs in DLD-1 cells (non-auxin treated) as well as HeLa cells (TorsinA/B/3A KO), both prepared by cryo-FIB. Median values from each distribution are indicated as a dotted line and below the plot. **** indicates a two-tailed p-value ≤ 0.0001 from Mann-Whitney U analysis. c, Measurements of NPC diameter from single NPCs in MEFs using cryo-FIB or nuclear envelope purification techniques. Median values from each distribution are indicated as a dotted line and below the plot. Representative tomographic slices from intact cells using cryo-FIB milling and from isolated NE preparations are shown. Slice thickness: 8.9 nm.
