Extended Data Fig. 6: Biochemical analysis of ShTnsC mutants.
From: Target site selection and remodelling by type V CRISPR-transposon systems
a, SDS-PAGE analysis of purified wild type and ATPase activity mutant TnsC proteins. b, Representative negative-stain EM micrographs of TnsC ATPase activity mutants incubated in the presence of dsDNA and ATP or AMPPNP. Magnification, 98,000x. Experiment was repeated twice independently with similar results. c, SDS-PAGE analysis of purified wild type and DNA binding mutant TnsC proteins. d, Representative negative stain EM micrographs of TnsC in the presence of AMPPNP and dsDNA and with mutations in the DNA binding interface. Magnification, 98,000x. Experiment was repeated twice independently with similar results. e, Electromobility shift assay using fluorophore-labeled 27-bp dsDNA in the presence of AMPPNP and either wild type or DNA binding mutant TnsC proteins. -, no protein control. Experiment was repeated twice independently with similar results. For gel source data, see Supplementary Fig. 1.
